An animal-free recombinant Factor C fluorescence assay for quantitative bacterial endotoxin detection.
The Lonza PyroGene® Recombinant Factor C Assay is an endpoint fluorescence method for detecting and quantifying Gram-negative bacterial endotoxin.
Endotoxin activates recombinant Factor C, which cleaves a fluorogenic substrate to generate a measurable fluorescence signal. Because the assay uses recombinant Factor C rather than horseshoe-crab lysate, it provides a non-animal-derived alternative for endotoxin testing.
Manufacturer: Lonza
Product line: PyroGene®
Catalog number: 50-658U
Test capacity: Up to 192 tests, depending on plate layout
Detection method: Endpoint fluorescence
Analyte: Bacterial endotoxin
Sensitivity: Down to approximately 0.005 EU/mL under appropriate assay conditions
Equipment required: Compatible fluorescence microplate reader
Storage: 2–8°C
Applications: Pharmaceutical quality control, raw-material testing, biologics testing and research endotoxin measurement
Regulatory suitability: Users must validate the method for their applicable regulatory workflow
Original product link: Lonza PyroGene Recombinant Factor C Assay
Contact Biolterna to confirm the required kit presentation, microplate reader compatibility and regulatory documentation.
Lonza lists 50-658U as the kit for up to 192 tests and states that the assay can detect endotoxin down to approximately 0.005 EU/mL.
