A high-fidelity Type IIS restriction enzyme optimized for Golden Gate Assembly.
BsaI-HF®v2 is an engineered Type IIS restriction endonuclease that recognizes an asymmetric DNA sequence and cleaves outside its recognition site. It is optimized for reliable Golden Gate Assembly and maintains strong activity in reaction conditions commonly used with T4 DNA Ligase. neb.com
Brand: NEB
Manufacturer: New England Biolabs
Catalog number: R3733
Product type: Type IIS restriction endonuclease
Recognition sequence: GGTCTC
Primary application: Golden Gate Assembly
Additional applications: Modular cloning and synthetic biology
Storage: −20°C
Research use only: Not for diagnostic procedures
Original product link: NEB official product page
Contact Biolterna for available unit sizes, Golden Gate workflow products, current availability or compatible Type IIS restriction enzymes.