A ligase-independent seamless cloning kit for rapid directional insertion of DNA fragments into linearized vectors.
Vazyme ClonExpress II One Step Cloning Kit is a homologous-recombination cloning system for inserting DNA fragments into linearized vectors without conventional ligation.
The insert is amplified with approximately 15–20 bp terminal sequences homologous to the vector ends. Following a 30-minute reaction at 37°C, the recombinant product can be transformed into competent cells.
Brand: Vazyme
Catalog number: C112-02
Pack size: 50 reactions, based on 20 μL reactions
Alternative size: 25 reactions
Product type: Seamless cloning kit
Cloning method: Ligase-independent homologous recombination
Compatible insert size: Approximately 50 bp to 10 kb for single-fragment cloning
Reported positive rate: Greater than 95% under suitable conditions
Reaction conditions: Approximately 30 minutes at 37°C
Storage: −30°C to −15°C
Research use only: Not for diagnostic procedures
Original product link: Vazyme ClonExpress II One Step Cloning Kit Vazyme Global
Contact Biolterna for current availability or assistance selecting a single- or multi-fragment cloning kit.
