A DNA-specific exonuclease for removing single-stranded primers and linear single-stranded DNA.
NEB Exonuclease I (E. coli) is a DNA-specific exonuclease that removes nucleotides from linear single-stranded DNA in the 3′→5′ direction. It is commonly used to degrade residual single-stranded primers following PCR while leaving double-stranded PCR products intact.
Typical applications include enzymatic PCR cleanup before Sanger sequencing, removal of primers before nested PCR and selective removal of linear single-stranded DNA. www.neb.com
Brand: NEB
Manufacturer: New England Biolabs
Catalog number: M0293
Product type: Single-stranded DNA exonuclease
Directionality: 3′→5′
Substrate preference: Linear single-stranded DNA
Applications: PCR cleanup, primer removal, Sanger sequencing preparation and SNP analysis
Storage: −20°C
Research use only: Not for diagnostic procedures
Original product link: NEB official product page
Contact Biolterna for available unit sizes, current lead time or alternative enzymatic PCR cleanup products.
