Recombinant wild-type Streptococcus pyogenes Cas9 nuclease for in-vitro DNA cleavage and guide-RNA screening.
GenScript GenCrispr Cas9 Nuclease is recombinant wild-type Cas9 protein produced in E. coli. It forms a ribonucleoprotein complex with guide RNA and introduces site-specific double-stranded breaks in DNA adjacent to an NGG PAM sequence.
This version does not contain a nuclear localization signal and is primarily suitable for in-vitro DNA cleavage, guide-RNA screening and antibody-production research.
Manufacturer: GenScript
Catalog number: Z03386-10
Pack size: 10 µg
Additional format: 50 µg, supplied as 5 × 10 µg
Protein source: Recombinant Streptococcus pyogenes Cas9 expressed in E. coli
Nuclear localization signal: None
PAM recognition: NGG
Applications: In-vitro DNA cleavage, guide-RNA efficiency screening and Cas9 antigen research
Storage: −20°C
Research use only: Not for therapeutic or diagnostic use
Original product link: GenScript GenCrispr Cas9 Nuclease Z03386
Contact Biolterna for this nuclease or NLS-containing Cas9 variants intended for cellular genome-editing workflows.
